Acute effects of swimming aerobic exercise on contractility and intracellular calcium handling in isolated right ventricular cardiomyocytes

Acute effects of aerobic exercise on contractility

  • Jean Carlos Loura dos Santos Physiology and Biochemistry Experimental Laboratory, Department of Sports, Center of Physical Education and Sports, Federal University of Espírito Santo, Vitória, ES Brazil
  • Erick Roberto Gonçalves Claudio (a) Physiology and Biochemistry Experimental Laboratory, Department of Sports, Center of Physical Education and Sports, Federal University of Espírito Santo, Vitória, ES Brazil. (b)Department of Physiological Sciences, Health Sciences Center, Federal University of Espírito Santo, Vitória, ES, Brazil.
  • Wellington Lunz Physiology and Biochemistry Experimental Laboratory, Department of Sports, Center of Physical Education and Sports, Federal University of Espírito Santo, Vitória, ES Brazil.
  • Elis Aguiar Morra Physiology and Biochemistry Experimental Laboratory, Department of Sports, Center of Physical Education and Sports, Federal University of Espírito Santo, Vitória, ES Brazil.
  • Vitor Loureiro da Silva Physiology and Biochemistry Experimental Laboratory, Department of Sports, Center of Physical Education and Sports, Federal University of Espírito Santo, Vitória, ES Brazil.
  • Jóctan Pimentel Cordeiro Physiology and Biochemistry Experimental Laboratory, Department of Sports, Center of Physical Education and Sports, Federal University of Espírito Santo, Vitória, ES Brazil.
  • Danilo Sales Bocalini Physiology and Biochemistry Experimental Laboratory, Department of Sports, Center of Physical Education and Sports, Federal University of Espírito Santo, Vitória, ES Brazil.
  • Ana Paula Lima Leopoldo Physiology and Biochemistry Experimental Laboratory, Department of Sports, Center of Physical Education and Sports, Federal University of Espírito Santo, Vitória, ES Brazil.
  • Andre S Leopoldo Physiology and Biochemistry Experimental Laboratory, Department of Sports, Center of Physical Education and Sports, Federal University of Espírito Santo, Vitória, ES Brazil.
DOI: https://doi.org/10.33155/j.ramd.2022.02.001
Keywords: ejercicio agudo, cardiomiocito, manejo de calcio, ventrículo derecho acute exercise, cardiomyocyte, calcium handling, right ventricle exercicio agudo, cadiomiocito, transiente de cálcio, ventrículo direito

Abstract

Aims: The acute adjustments on the contractile function and the intracellular calcium (Ca2+) handling in the cardiomyocytes of the right ventricle (RV) after an acute aerobic exercise session are not known. Our aim was to investigate the acute responses of the contractile function and the intracellular Ca2+ handling in isolated RV cardiomyocytes after a swimming exercise session.

Methods: Ten-week-old female Wistar rats were randomly allocated into two groups: control (C; n = 5) and exercise (Ex; n = 7). It was performed a swimming exercise session for 30 min. with an overload of 4% relative to the body weight attached to the tail. The rats were sacrificed after the exercise session for the analysis of the RV contractile function parameters and the intracellular Ca2+ handling by the isolated cardiomyocyte technique.

Results: Body and heart weights, as well as sarcomere length were similar between the groups. Also, it was not observed differences between the groups for RV cardiomyocyte contractile parameters. However, the systolic and diastolic intracellular Ca2+ concentration was significantly lower in the Ex group compared to C with maintenance of Ca2+ amplitude.

Conclusion: An acute swimming aerobic exercise session promotes cardiomyocyte contractility maintenance even with systolic and diastolic intracellular Ca2+ concentration reduced in the RV cardiomyocytes, reflecting an improvement in the intracellular Ca2+ handling.

Published
2022-02-14
Issue
Vol. 15 No. 1 (2022): March 2022
Section
Originals
Page/s
12-17
DOI
https://doi.org/10.33155/j.ramd.2022.02.001